Anti-Cdc25A (phospho S178) 抗体 (ab79252)
Key features and details
- Rabbit polyclonal to Cdc25A (phospho S178)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Cdc25A (phospho S178) antibody
Cdc25A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cdc25A (phospho S178) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human Cdc25A (phospho S178).
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ポジティブ・コントロール
- WB: extracts from UV-treated A549 cells; IHC-P: Human breast carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab79252の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 59 kDa.
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IHC-P |
1/50 - 1/100.
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特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 59 kDa. |
IHC-P
1/50 - 1/100. |
ターゲット情報
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機能
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro. -
配列類似性
Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain. -
ドメイン
The phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction. -
翻訳後修飾
Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation.
Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization. - Information by UniProt
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参照データベース
- Entrez Gene: 993 Human
- Entrez Gene: 12530 Mouse
- Entrez Gene: 171102 Rat
- Omim: 116947 Human
- SwissProt: P30304 Human
- SwissProt: P48964 Mouse
- SwissProt: P48965 Rat
- Unigene: 437705 Human
see all -
別名
- Cdc 25a antibody
- CDC25A antibody
- CDC25A2 antibody
see all
画像
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Left panel: immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue using 1/50 ab79252. Right panel: same, after pre-incubation with the immunizing peptide.
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All lanes : Anti-Cdc25A (phospho S178) antibody (ab79252) at 1/500 dilution
Lane 1 : Extracts from A549 cell treated
with UV (15mins)
Lane 2 : Extracts from A549 cell treated
with UV (15mins) with immunizing phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 59 kDa
Observed band size: 59 kDa
Additional bands at: 110 kDa, 117 kDa. We are unsure as to the identity of these extra bands.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab79252 は 2 報の論文で使用されています。
- Liu WH et al. LINC00473 contributes to the radioresistance of esophageal squamous cell carcinoma by regulating microRNA-497-5p and cell division cycle 25A. Int J Mol Med 46:571-582 (2020). PubMed: 32468021
- Xu X et al. A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity. Nat Commun 8:14059 (2017). WB ; Human . PubMed: 28094252