Application
Western blot
Sample
Mouse Cell lysate - whole cell (C2C12 myoblast/myotube)
Loading amount
5 µg
Specification
C2C12 myoblast/myotube
Treatment
Differentiation medium, 0-72 hours
Gel Running Conditions
Reduced Denaturing (10-15%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 3% BSA/TBS-Tween
Secondary antibody
Name
Non-Abcam antibody was used: Anti rabbit HRP conjugate
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Exposure
20 second(s)
Bands
Specific: 21 kDa
Additional data
PubMed ID
19481146: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Hilary Pollard
Verified customer
投稿 May 20 2011