Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293, Kidney)
Loading amount
100 µg
Specification
HEK 293, Kidney
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C
Secondary antibody
Name
Non-Abcam antibody was used: Pierce
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/5000
Detection
Detection method
ECL
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 22 kDa Non-specific: 31/48/60 kDa
Additional data
Additional Notes
The antibody can stain the CAV-2 if expose longer / load more protein/ stain more abs. However, the strong 31kd non-specific band may prevent it from using in Immunofluorescence.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Olga Roche
Verified customer
投稿 Dec 19 2007