Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (A7r5 (rat smooth muscle cells))
Specification
A7r5 (rat smooth muscle cells)
Fixative
Paraformaldehyde
Permeabilization
Yes - Saponine 0,1%
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/50
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA 1% + Saponine 0,1%
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor® 488 donkey anti-goat IgG (H+L) 2 mg/m
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/300
Additional data
Abcam response
Thank you for your valuable feedback on this antibody in the untested application ICC/IF. Based on the results here, this antibody may not be suitable for use in this application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Cyril Mougin
Verified customer
投稿 Mar 24 2010