Application
Immunocytochemistry
Sample
Cow Cultured Cells (Bovine brain microvascular endothelial cells)
Specification
Bovine brain microvascular endothelial cells
Fixative
Paraformaldehyde
Permeabilization
Yes - Saponine 0.1% in PBS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Other product details
Dilution
1/20
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA1% in Saponine 0.1%-PBS
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor® 568 goat anti-rabbit IgG (H+L) 2 mg/m
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Dilution
1/300
Additional data
Abcam response
Thank you for your valuable feedback. We are sorry to hear that this antibody has not given you good results in immunocytochemistry using bovine cells.
This antibody has not been tested in this species before, so we would recommend running a positive control along with the samples to check its suitability. According to the expression profile of this protein, brain tissue give a negative results in antibody staining level. Instead, Liver sinusoids, bile duct cells, placenta, heart and smooth muscle showed moderate cytoplasmic and membranous immunoreactivity.
We would welcome more feedback from our customers in order to make further decision about the species suitability.
This antibody has not been tested in this species before, so we would recommend running a positive control along with the samples to check its suitability. According to the expression profile of this protein, brain tissue give a negative results in antibody staining level. Instead, Liver sinusoids, bile duct cells, placenta, heart and smooth muscle showed moderate cytoplasmic and membranous immunoreactivity.
We would welcome more feedback from our customers in order to make further decision about the species suitability.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Cyril Mougin
Verified customer
投稿 Jun 22 2012