Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma)
Loading amount
50 µg
Specification
Glioblastoma
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 30°C
Other product details
Dilution
1/750
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% Milk in TBS
Secondary antibody
Name
Non-Abcam antibody was used: anti mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
DAB
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 26, 34, 42 kDa
Positive control
U87MG cell lysate
Additional data
Additional Notes
membrane used: NCM
Lysis buffer used: RIPA
Lysis buffer used: RIPA
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Ratnakar Singh
Verified customer
投稿 Jun 10 2011