Anti-Cathepsin F 抗体 (ab36161)
Key features and details
- Goat polyclonal to Cathepsin F
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Cathepsin F antibody
Cathepsin F 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Cathepsin F -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Dog -
免疫原
Synthetic peptide:
CGVNTMASSAVVD
, corresponding to C terminal amino acids 472-484 of Human Cathepsin F -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab36161の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
特記事項 |
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WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 53 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. -
組織特異性
High expression levels in heart, skeletal muscle, brain, testis and ovary; moderate levels in prostate, placenta, liver and colon; and no detectable expression in peripheral leukocytes and thymus. -
配列類似性
Belongs to the peptidase C1 family. -
細胞内局在
Lysosome. - Information by UniProt
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参照データベース
- Entrez Gene: 8722 Human
- Entrez Gene: 56464 Mouse
- Omim: 603539 Human
- SwissProt: Q9UBX1 Human
- SwissProt: Q9R013 Mouse
- Unigene: 11590 Human
- Unigene: 29561 Mouse
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別名
- AI481912 antibody
- CATF_HUMAN antibody
- Cathepsin F antibody
see all
画像
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Anti-Cathepsin F antibody (ab36161) at 0.3 µg/ml + Human Ovary lysate (35µg protein in RIPA buffer).
Predicted band size: 53 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.Experiments gave bands at approx 30kDa and 35kDa in Human Prostate and Ovary lysates after 0.3µg/ml antibody staining. These bands correspond to earlier findings in literature (Wang et al, J Biol Chem. 1998 Nov 27;273(48):32000-8; PMID: 9822672). This protein has a calculated MW of 53.4kDa according to NP_003784.2.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab36161 は 1 報の論文で使用されています。
- Jeric B et al. N-terminally truncated forms of human cathepsin F accumulate in aggresome-like inclusions. Biochim Biophys Acta 1833:2254-66 (2013). Human . PubMed: 23684953