Application
Western blot
Sample
Human Cell lysate - whole cell (blood monocyte derived macrophage)
Loading amount
100 µg
Specification
blood monocyte derived macrophage
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/2500
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odessey Li-cor BLOCKING BUFFER
Secondary antibody
Name
Non-Abcam antibody was used: (H+L) DyLight 680 Dye conjugated goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight® 650
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight® 650
Dilution
1/10000
Detection
Detection method
LI-COR Odyssey* Infrared Imaging System.
Exposure
1 second(s)
Bands
Specific: 46 and 28 kDa
Positive control
no controls
Negative control
no controls
Additional data
Additional Notes
The main band comes for the heavy chain at 28 kda. This must be mentioned in the datasheet. In human macrophages the heavy chain is mainly expressed. We can also find the 46 kda band. There is a FAINT non-specific band at around 15 kda. However, this might be a cleaved product arising from some unknown cleavage.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 18 2013