Alexa Fluor® 594 Anti-Cathepsin D 抗体 [EPR3057Y] (ab207874)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 594 Rabbit monoclonal [EPR3057Y] to Cathepsin D
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 594. Ex: 590nm, Em: 617nm
Related conjugates and formulations
製品の概要
-
製品名
Alexa Fluor® 594 Anti-Cathepsin D antibody [EPR3057Y]
Cathepsin D 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 594 Rabbit monoclonal [EPR3057Y] to Cathepsin D -
由来種
Rabbit -
標識
Alexa Fluor® 594. Ex: 590nm, Em: 617nm -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- ICC/IF: MCF7 cells
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3057Y -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
- Alexa Fluor® 488 Anti-Cathepsin D antibody [EPR3057Y] (ab197605)
- Alexa Fluor® 647 Anti-Cathepsin D antibody [EPR3057Y] (ab198326)
- Anti-Cathepsin D antibody [EPR3057Y] - BSA and Azide free (ab207549)
- Alexa Fluor® 405 Anti-Cathepsin D antibody [EPR3057Y] (ab207875)
- PE Anti-Cathepsin D antibody [EPR3057Y] (ab305650)
- APC Anti-Cathepsin D antibody [EPR3057Y] (ab305651)
- HRP Anti-Cathepsin D antibody [EPR3057Y] (ab305652)
- Anti-Cathepsin D antibody [EPR3057Y] (ab75852)
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab207874の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
1/100.
This product gave a positive signal in MCF7 cells fixed with 100% methanol (5 min) |
特記事項 |
---|
ICC/IF
1/100. This product gave a positive signal in MCF7 cells fixed with 100% methanol (5 min) |
ターゲット情報
-
機能
Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. -
組織特異性
Expressed in the aorta extrcellular space (at protein level). -
関連疾患
Ceroid lipofuscinosis, neuronal, 10 -
配列類似性
Belongs to the peptidase A1 family.
Contains 1 peptidase A1 domain. -
翻訳後修飾
N- and O-glycosylated. -
細胞内局在
Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380). - Information by UniProt
-
参照データベース
- Entrez Gene: 1509 Human
- Entrez Gene: 13033 Mouse
- Omim: 116840 Human
- SwissProt: P07339 Human
- SwissProt: P18242 Mouse
- Unigene: 654447 Human
-
別名
- CatD antibody
- CATD_HUMAN antibody
- Cathepsin D antibody
see all
画像
-
ab207874 staining Cathepsin D in MCF7 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab207874 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab207874 は論文での使用が確認できていません。