Alexa Fluor® 405 Anti-Cathepsin D 抗体 [EPR3057Y] (ab207875)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 405 Rabbit monoclonal [EPR3057Y] to Cathepsin D
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: Alexa Fluor® 405. Ex: 402nm, Em: 421nm
Related conjugates and formulations
製品の概要
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製品名
Alexa Fluor® 405 Anti-Cathepsin D antibody [EPR3057Y]
Cathepsin D 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 405 Rabbit monoclonal [EPR3057Y] to Cathepsin D -
由来種
Rabbit -
標識
Alexa Fluor® 405. Ex: 402nm, Em: 421nm -
アプリケーション
適用あり: Flow Cyt (Intra)more details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Flow Cyt (intra): MCF7 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3057Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Alexa Fluor® 488 Anti-Cathepsin D antibody [EPR3057Y] (ab197605)
- Alexa Fluor® 647 Anti-Cathepsin D antibody [EPR3057Y] (ab198326)
- Anti-Cathepsin D antibody [EPR3057Y] - BSA and Azide free (ab207549)
- Alexa Fluor® 594 Anti-Cathepsin D antibody [EPR3057Y] (ab207874)
- PE Anti-Cathepsin D antibody [EPR3057Y] (ab305650)
- APC Anti-Cathepsin D antibody [EPR3057Y] (ab305651)
- HRP Anti-Cathepsin D antibody [EPR3057Y] (ab305652)
- Anti-Cathepsin D antibody [EPR3057Y] (ab75852)
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab207875の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/50.
The cellular localisation of this product has been verified in ICC/IF. |
特記事項 |
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Flow Cyt (Intra)
1/50. The cellular localisation of this product has been verified in ICC/IF. |
ターゲット情報
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機能
Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. -
組織特異性
Expressed in the aorta extrcellular space (at protein level). -
関連疾患
Ceroid lipofuscinosis, neuronal, 10 -
配列類似性
Belongs to the peptidase A1 family.
Contains 1 peptidase A1 domain. -
翻訳後修飾
N- and O-glycosylated. -
細胞内局在
Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380). - Information by UniProt
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参照データベース
- Entrez Gene: 1509 Human
- Entrez Gene: 13033 Mouse
- Omim: 116840 Human
- SwissProt: P07339 Human
- SwissProt: P18242 Mouse
- Unigene: 654447 Human
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別名
- CatD antibody
- CATD_HUMAN antibody
- Cathepsin D antibody
see all
画像
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Overlay histogram showing MCF7 cells stained with ab207875 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were blocked with Image-iT® FX Signal Enhancer for 30 min at 22°C and then blocked with 1x PBS / 10% normal goat serum followed by the antibody (ab207875, 1/50 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 405 (ab208150) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Violet laser (405nm) and 450/50 bandpass filter.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab207875 は論文での使用が確認できていません。