Anti-Cathepsin B 抗体 [CA10] (ab58802)
Key features and details
- Mouse monoclonal [CA10] to Cathepsin B
- Suitable for: Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-Cathepsin B antibody [CA10]
Cathepsin B 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [CA10] to Cathepsin B -
由来種
Mouse -
特異性
This antibody recognizes an epitope on the heavy chain of mature Cathepsin B and therefore detects both mature (~31 kDa) and pro (~43 kDa)Cathepsin B. -
アプリケーション
適用あり: Flow Cyt, WBmore details -
種交差性
交差種: Human -
免疫原
Cathepsin B purified from human liver
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エピトープ
Within the heavy chain of mature cathepsin B -
ポジティブ・コントロール
- Flow cyt: HepG2 cells
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特記事項
This product has switched from ascites to TCS on 26th October 2020.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Constituents: 0.158% Ammonium bicarbonate, 0.1% BSA -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
CA10 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab58802の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
Use 0.5µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
WB | (4) |
Use a concentration of 0.25 µg/ml. Predicted molecular weight: 38 kDa.
|
特記事項 |
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Flow Cyt
Use 0.5µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 0.25 µg/ml. Predicted molecular weight: 38 kDa. |
ターゲット情報
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機能
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. -
配列類似性
Belongs to the peptidase C1 family. -
細胞内局在
Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 1508 Human
- Omim: 116810 Human
- SwissProt: P07858 Human
- Unigene: 520898 Human
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別名
- Amyloid precursor protein secretase antibody
- APP secretase antibody
- APPS antibody
see all
画像
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Anti-Cathepsin B antibody [CA10] (ab58802) at 0.25 µg/ml +
Native human Cathepsin B protein (ab90387) at 0.01 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Exposure time: 10 seconds -
Overlay histogram showing HepG2 cells stained with ab58802 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58802, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (84)
ab58802 は 84 報の論文で使用されています。
- Nishtala K et al. Quantitative Proteomics Reveals Molecular Network Driving Stromal Cell Differentiation: Implications for Corneal Wound Healing. Int J Mol Sci 23:N/A (2022). PubMed: 35269714
- Sugimoto T et al. Investigation of Brain Function-Related Myokine Secretion by Using Contractile 3D-Engineered Muscle. Int J Mol Sci 23:N/A (2022). PubMed: 35628536
- Liu Y et al. Curcumin Elevates microRNA-183-5p via Cathepsin B-Mediated Phosphatidylinositol 3-Kinase/AKT Pathway to Strengthen Lipopolysaccharide-Stimulated Immune Function of Sepsis Mice. Contrast Media Mol Imaging 2022:6217234 (2022). PubMed: 35992541
- Alegre-Cortés E et al. Delay of EGF-Stimulated EGFR Degradation in Myotonic Dystrophy Type 1 (DM1). Cells 11:N/A (2022). PubMed: 36230978
- Lucchino V et al. Insights into the Genetic Profile of Two Siblings Affected by Unverricht-Lundborg Disease Using Patient-Derived hiPSCs. Cells 11:N/A (2022). PubMed: 36359887