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Products:Microbiology >> Lipopolysaccharide >> Bacterial Lipopolysaccharide
Overview
Product name
Anti-Cathelicidin antibody
Cathelicidin 抗体 (14件) 一覧
Description
Rabbit polyclonal to Cathelicidin
Specificity
ab64892 is raised against the LL37 region and will recognize both the full length and cleaved C terminus.
Tested applications
ICC/IF, WB, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Human
Immunogen
A synthetic peptide as a part of human Cathelicidin conjugated to an immunogenic carrier protein. Peptide available as ab96020.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Secreted Molecules >> Other secreted molecules
Microbiology >> Lipopolysaccharide >> Bacterial Lipopolysaccharide
Applications
Show applications key
Our Abpromise guarantee covers the use of ab64892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
WB: 1/100 - 1/2000.Predicted molecular weight: 19 kDa.
IHC-P: 1/100 - 1/2000.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr
IHC-Fr: 1/100 - 1/2000.IHC-Fr: 1/100 - 1/2000.
Target
Function
Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity.
Tissue specificity
Expressed in bone marrow and testis and neutrophils.
Sequence similarities
Belongs to the cathelicidin family.
Post-translational
modifications
The N-terminus is blocked.
Cellular localization
Secreted.
Target information above from: UniProt accessionP49913
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 18 kDa cationic antimicrobial protein antibody
- Antibacterial protein FALL-39 antibody
- Antibacterial protein LL-37 antibody
see all
Database links
- Entrez Gene: 12796 Human
- Entrez Gene: 820 Human
- Omim: 600474 Human
- SwissProt: P49913 Human
- Unigene: 51120 Human
Anti-Cathelicidin antibody images:
Immunocytochemistry/ Immunofluorescence - Cathelicidin antibody (ab64892)
ab64892 staining a cytospin preparation of peripheral blood mononuclear cells isolated from buffycoat. Cells were left to air dry, fixed with cold acetone (90 secs) and blocked in PBS, 1%FCS and 0.1% saponin for 20 mins. Incubation with ab64892 (1/200) was done for 1 hour at room temperature. Cells were washed 2x PBS, incubated with a FITC-labelled secondary antibody, washed in PBS and counterstained with DAPI. ab64892 selectively recognizes polymorphonuclar cells.
Western blot - Cathelicidin antibody (ab64892)
Lane 1 : Anti-Cathelicidin antibody (ab64892) at 1/100 dilution
Lane 2 : Anti-Cathelicidin antibody (ab64892) at 1/100 dilution (preabsorbed with immunising peptide)
Lane 1 : Whole cell lysate prepared from Human neutrophils
Lane 2 : Whole cell lysate prepared from Human neutrophils
Predicted band size : 19 kDa
Immunocytochemistry/ Immunofluorescence - Cathelicidin antibody (ab64892)
ab64892 (1/100 in whole serum) staining cathelicidin in ethanol-fixed human spermatozoa.
References for Anti-Cathelicidin antibody (ab64892)
This product has been referenced in:
- Braun BJet al. The formyl peptide receptor like-1 and scavenger receptor MARCO are involved in glial cell activation in bacterial meningitis. J Neuroinflammation 8:11 (2011). ICC/IF; Rat.Read more (PubMed: 21299846) »
- Gambichler Tet al. Significant upregulation of antimicrobial peptides and proteins in lichen sclerosus. Br J Dermatol 161:1136-42 (2009). IHC-P; Human.Read more (PubMed: 19558556) »
See all 2 publications for this product
Publishing research using ab64892? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"