Application
Western blot
Sample
Human Tissue lysate - whole (Human Penile Tissue)
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Loading amount
60 µg
Specification
Human Penile Tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3 % milk
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: ECL Donkey anti-rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Chemiluminescence, ECL
Negative control
I did not have a negative control
Exposure
5 second(s)
Bands
Specific: 60 kDa
Positive control
I did not have a positive control
Additional data
Additional Notes
I would decrease the concentration of the primary antibody the next time I used this because I got strong bands in both human and mouse tissue after only 5 seconds exposure
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Miss. Gwen Lagoda
Verified customer
投稿 Dec 30 2011