Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
Yes - 0.2% Triton x-100
Specification
Liver
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
9 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/200
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Alexa Fluor 488 goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
DAPI for staining nuclei
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投稿 Nov 25 2010