Rabbit monoclonal [E180] to Caspase-6
This antibody detects the pro form and the p11 subunit of human Caspase-6. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Synthetic peptide within Human Caspase-6 aa 250-350 (C terminal). The exact sequence is proprietary.
Database link: P55212
WB: Jurkat cell lysate.
ICC/IF: Hela cells.
IHC-P: Human lung carcinoma tissue slides.
A trial size is available to purchase for this antibody.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa).
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for IP.
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
Belongs to the peptidase C14A family.
Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.
Information by UniProt
Apoptotic protease Mch-2 antibody
Apoptotic protease MCH2 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6 antibody [E180] (ab32366)
Ab32366, at a 1:50 dilution, staining Caspase-6 in paraffin embedded human lung adenocarcinoma tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-Caspase-6 antibody [E180] (ab32366)
Ab32366, at a 1:100 dilution, staining Caspase-6 in Hela cells by Immunocytochemistry/Immunofluorescence.
Western blot - Anti-Caspase-6 antibody [E180] (ab32366)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"