Application
Western blot
Sample
Human Cell lysate - whole cell (Keratinocytes)
Loading amount
20 µg
Specification
Keratinocytes
Gel Running Conditions
Reduced Denaturing (4~12% SDS PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/3000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBST
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 27 kDa Non-specific: 50 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. HYOUNG JUNE KIM
Verified customer
投稿 Oct 26 2009