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Our Abpromise guarantee covers the use of ab2012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).Can be blocked with Human Caspase-10 peptide (ab8377).|
|ICC/IF||1/50. Fix with PFA.|
ab2012 at 1:50 staining cultured mouse hippocampal astrocytes by ICC/IF. The astrocytes were maintained in culture for 2 days in the presence of 10% serum, fixed with 4% PFA in PBS for 15 min at room temperature and permeabilized for 5 min in 0.25% Triton X-100 in PBS. The tissue was incuabated with the antibody for 2 hours at room temperature. A Cy3 ® conjugated donkey anti-rabbit IgG (H+L) was used as the secondary.
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