Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (U2OS cells)
Total protein in input
500 µg
Immuno-precipitation step
Protein A/G
Specification
U2OS cells
Other product details
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Lysis buffer
Concentration
10 µg/mg lysate
Western Blot antibody
Dilution
1/1000
Name
Abcam antibody:Anti-Casein Kinase 2 beta antibody [EP1995Y]
Additional data
Additional Notes
500 ug of whole cell lysates from U2OS cells were incubated with 5 ug of antibody for 3 hours at 4 degree. Then, protein A/G magnetic beads were added and incubated at 4 degree for 2 hours.
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投稿 May 12 2017