Application
Western blot
Sample
Cow Tissue lysate - whole (Heart tissue)
Loading amount
20 µg
Specification
Heart tissue
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Other product details
Dilution
1/2000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS 3% marvel milk
Secondary antibody
Name
Non-Abcam antibody was used: DAKO goat anti-mouse HRP
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 25 kDa
Negative control
cow skeletal muscle
Additional data
Additional Notes
The antibody has also been used to detect pig and sheep cTnI in heart extracts by Western blotting.
Lane 1 - cow skeletal muscle extract
Lane 2 - cow heart extract
Lane 1 - cow skeletal muscle extract
Lane 2 - cow heart extract
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 15 2010