Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Heart)
Antigen retrieval step
None
Permeabilization
No
Specification
Heart
Blocking step
Serum free as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 0µg/mL · Temperature: 24°C
Fixative
Formaldehyde
Other product details
Incubation time
60 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: TBSTween
Concentration
4 µg/ml
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Biotinylated rabbit anti-mouse Igs
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Positive staining throughout myocardium after immunohistochemistry. Primary omission generated clear staining. Western blot analysis revealed band at 14kDa (same region as target protein) but this was also observed in isotype control. Thus, inconclusive specificity.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Feb 26 2009