Anti-CEACAM5 + CEACAM6 抗体 [NCRC16 (AKA 161)] (ab22705)
Key features and details
- Mouse monoclonal [NCRC16 (AKA 161)] to CEACAM5 + CEACAM6
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-CEACAM5 + CEACAM6 antibody [NCRC16 (AKA 161)] -
製品の詳細
Mouse monoclonal [NCRC16 (AKA 161)] to CEACAM5 + CEACAM6 -
由来種
Mouse -
特異性
Cross reacts with a similar epitope on NCA. -
アプリケーション
適用あり: ICC/IF, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Colorectal cancer cell lines (Human).
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ポジティブ・コントロール
- LS174 T cells, colon carcinoma IHC-P: FFPE human colon tissue sections.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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精製度
Protein A/G purified -
ポリ/モノ
モノクローナル -
クローン名
NCRC16 (AKA 161) -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab22705の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 1 µg/ml.
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IHC-P |
Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ICC/IF
Use a concentration of 1 µg/ml. |
IHC-P
Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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細胞内局在
CEACAM5: Cell membrane. CEACAM6: Cell membrane. -
参照データベース
- Entrez Gene: 1048 Human
- Entrez Gene: 4680 Human
- Omim: 114890 Human
- Omim: 163980 Human
- SwissProt: P06731 Human
- SwissProt: P40199 Human
- Unigene: 466814 Human
- Unigene: 709196 Human
画像
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IHC image of Carcino Embryonic Antigen CEA staining in human colon formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22705, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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ICC/IF image of ab22705 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22705, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab22705 は 1 報の論文で使用されています。
- Durrant LG et al. Enhanced recognition of human colorectal tumour cells using combinations of monoclonal antibodies. Br J Cancer 60:855-60 (1989). PubMed: 2481485