Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (intervertebral disc)
Specification
intervertebral disc
Fixative
Formaldehyde
Antigen retrieval step
Enzymatic
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10 %
Other product details
Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Vectastain ABC anti-mouse sec.
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Abcam response
Thank you for your feedback. We hope the troubleshooting suggestions we made have improved your results with this antibody.
We would recommend trying the antibody at 1:100. I see from the datasheet that this antibody is supplied at 0.25 ug/ml, and the concentration recommended is 2 ug/ml, which would be 1/125. With some antibodies, 30 minutes of blocking with 10% NS is adequate.
We would recommend trying the antibody at 1:100. I see from the datasheet that this antibody is supplied at 0.25 ug/ml, and the concentration recommended is 2 ug/ml, which would be 1/125. With some antibodies, 30 minutes of blocking with 10% NS is adequate.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 03 2007