Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Soft Tissue Sarcoma, Gastric Mucosa)
Specification
Soft Tissue Sarcoma, Gastric Mucosa
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Other product details
Dilution
1/1500
Incubation time
15 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Donkey Anti-Rabbit IgG (H+L)
Host species: Donkey
Clonality: Monoclonal
Conjugation: Biotin
Host species: Donkey
Clonality: Monoclonal
Conjugation: Biotin
Additional data
Additional Notes
I used a standard citrate buffer in a black and decker food steamer for 20 mintues for anitgen retrieval. This gave nice retrieval without damaging my tissue.
For western blot I loaded a total of 10 ug of protein and used the ab at a concentration of 1:2000. I used an Abcam goat anti-rabbit HRP secondary for western blot at a dilution of 1:5000
For western blot I loaded a total of 10 ug of protein and used the ab at a concentration of 1:2000. I used an Abcam goat anti-rabbit HRP secondary for western blot at a dilution of 1:5000
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 13 2006