Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Sample
Human Tissue sections (Colorectal tumors)
Specification
Colorectal tumors
Permeabilization
Yes - 1xPBS+0.3%t-X-100
Fixative
Formaldehyde
Other product details
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: 1xPBS+0.3%t-X-100+1%BSA
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rabbit-Alexa-488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/2000
Additional data
Additional Notes
Good for detecting hypoxic areas in CRC tumors: it showed that CA1X expression was very high in tumor cell areas than in stromal areas.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 09 2014