Anti-Carbonic Anhydrase 9/CA9 抗体 [2D3] (ab107257)
Key features and details
- Mouse monoclonal [2D3] to Carbonic Anhydrase 9/CA9
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Carbonic Anhydrase 9/CA9 antibody [2D3]
Carbonic Anhydrase 9/CA9 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2D3] to Carbonic Anhydrase 9/CA9 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human Carbonic Anhydrase 9/CA9.
Database link: Q16790 -
ポジティブ・コントロール
- Hela and A549 cell lysate; lung and colonic tissues and NTERA-2 cells.
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特記事項
This product was changed from ascites to supernatant. Lot no’s high than GR311638-1 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
2D3 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab107257の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
WB | (1) |
1/500 - 1/2000. Predicted molecular weight: 50 kDa.
|
IHC-P | (1) |
1/200 - 1/1000.
|
特記事項 |
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Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
1/500 - 1/2000. Predicted molecular weight: 50 kDa. |
IHC-P
1/200 - 1/1000. |
ターゲット情報
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機能
Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. -
組織特異性
Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. -
配列類似性
Belongs to the alpha-carbonic anhydrase family.
Contains 1 alpha-carbonic anhydrase domain. -
翻訳後修飾
Asn-346 bears high-mannose type glycan structures. -
細胞内局在
Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus. - Information by UniProt
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参照データベース
- Entrez Gene: 768 Human
- Omim: 603179 Human
- SwissProt: Q16790 Human
- Unigene: 63287 Human
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別名
- CA-IX antibody
- CA9 antibody
- CAH9_HUMAN antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded lung tissues (left) and colonic tissues (right) using 1/200 ab107257 with DAB staining.
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All lanes : Anti-Carbonic Anhydrase 9/CA9 antibody [2D3] (ab107257) at 1/500 dilution
Lane 1 : Hela Cell lysate
Lane 2 : A549 Cell Lysate
Predicted band size: 50 kDa -
Flow cytometric analysis of NTERA-2 cells using Carbonic Anhydrase 9/CA9 mouse ab107257 in green and negative control in purple.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab107257 は 8 報の論文で使用されています。
- Mishra CB et al. Anti-breast cancer action of carbonic anhydrase IX inhibitor 4-[4-(4-Benzo[1,3]dioxol-5-ylmethyl-piperazin-1-yl)-benzylidene-hydrazinocarbonyl]-benzenesulfonamide (BSM-0004): in vitro and in vivo studies. J Enzyme Inhib Med Chem 36:954-963 (2021). PubMed: 33947294
- Chen W et al. Identification of anaplastic lymphoma kinase fusions in clear cell renal cell carcinoma. Oncol Rep 43:817-826 (2020). PubMed: 32020234
- Suri BK et al. Carbonic anhydrases in human keratinocytes and their regulation by all-trans retinoic acid and 1a,25-dihydroxyvitamin D3. Exp Dermatol 28:976-980 (2019). PubMed: 31125456
- Brahmer A et al. Platelets, endothelial cells and leukocytes contribute to the exercise-triggered release of extracellular vesicles into the circulation. J Extracell Vesicles 8:1615820 (2019). PubMed: 31191831
- Yang WJ et al. Overexpression of Tie2 is associated with poor prognosis in patients with gastric cancer. Oncol Lett 15:8027-8033 (2018). PubMed: 29849805
- Sun W et al. Interaction between von Hippel-Lindau Protein and Fatty Acid Synthase Modulates Hypoxia Target Gene Expression. Sci Rep 7:7190 (2017). PubMed: 28775317
- Amiri A et al. Inhibition of carbonic anhydrase IX in glioblastoma multiforme. Eur J Pharm Biopharm 109:81-92 (2016). PubMed: 27702686
- Schweiger T et al. Carbonic anhydrase IX is associated with early pulmonary spreading of primary colorectal carcinoma and tobacco smoking. Eur J Cardiothorac Surg N/A:N/A (2013). Human . PubMed: 24321995