Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (12.5% gel)
Sample
Human Cell lysate - whole cell (293T)
Specification
293T
Treatment
CA2-3xflag transfected
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 4% milk
Dilution
1/5000
Secondary antibody
Name
Non-Abcam antibody was used: anti-Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Dilution
1/10000
Detection
Detection method
ECL
Bands
Specific: 26, 30 kDa
Exposure
1 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 30 2015