Application
Western blot
Sample
Mouse Tissue lysate - other (mouse brain - post nuclear supernatant)
Loading amount
20 µg
Specification
mouse brain - post nuclear supernatant
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS 0,1% Tween-20
Secondary antibody
Name
Non-Abcam antibody was used: Peroxidase goat anti rabbit IgG-antibody PI-1000 V
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL+
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: ~60 kDa Non-specific: several kDa
Additional data
Additional Notes
Excising the area with the expected bands from the Blots increases detection efficiency.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 05 2013