Application
Western blot
Sample
Mouse Tissue lysate - whole (heart, ventricular tissue)
Loading amount
60 µg
Specification
heart, ventricular tissue
Gel Running Conditions
Reduced Denaturing (4~12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Dilution
1/5000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 10mM Na phosphate (pH7.2), 0.02% Tween20
Secondary antibody
Name
Non-Abcam antibody was used: Jackson ImmunoResearch
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
Pierce SuperSignal
Exposure
10 second(s)
Bands
Specific: 56, 54 kDa Non-specific: 28 kDa
Positive control
ventricular tissue (my sample)
Negative control
none
Additional data
Additional Notes
PVDF membrane
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Feb 02 2008