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Product Name
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Calretinin antibody, prediluted
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Calretinin 抗体 (14件) 一覧
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Calretinin, prediluted
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Immunogen
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Recombinant full length mouse Calretinin protein.
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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IHC-Fr, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
We suggest an incubation period of 30 minutes at room temperature or overnight at 4 degrees Celsius. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Mesothelioma.
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Cellular localization
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Cytoplasmic and Nuclear
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Research areas
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Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Binding Proteins
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Relevance
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Calretinin is a 29 kDa calcium binding protein that is expressed in central and peripheral nervous system and in many normal and pathological tissues. Calretinin is also considered an important diagnostic aid in the differential diagnosis of cystic and solid ameloblastic tumors. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Calretinin is generally expressed with two other calcium binding proteins Calbindin D-29 and parvalbumin in CNS and other peripheral tissues. Two calcium binding proteins, calbindin and calretinin, have been reported to be expressed in abundance in Purkinje cells and other cell types in the cerebellum. The antibodies against calretinin also labels the mossy fibers throughout, and climbing fibers in a distinct group of folia. They also stain several cell types in the molecular layer. There is no appreciable immunostaining of calretinin in cerebellar cortex. In the rat, antibodies against calretinin stain the Lugaro cells, and some granule cells in lobe X; they also give weak staining of all the granule cells in the other lobes. Calretinin antibodies have been used to detect a number of carcinomas by clinicopathological, immunohistochemical techniques including, epithelial malignant mesotheliomas, Ovarian yolk sac tumor, Adenomatoid tumors of the uterus etc.
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Calretinin antibody, prediluted (ab7286)の別名 |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Constituents: PBS
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Form
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Liquid
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Storage instructions
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Store at +4°C.
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