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Anti-Calretinin antibody (ab702)

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Overview

Product name

Anti-Calretinin antibody
Calretinin 抗体 (15件) 一覧

Description

Rabbit polyclonal to Calretinin

Tested applications

IHC-P, IHC-Frmore details

Cross reactivity

Reacts with

Mouse, Rat, Chicken, Cat, Human, Xenopus laevis, Zebrafish

Immunogen

Full length protein

Positive control

Mesothelioma.

General notes

Contains a proprietary preservative that is not sodium azide or thimerosal.

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: PBS, Carrier protein, Van Gogh Yellow Diluent, pH 7.3

Purity

Whole antiserum

Clonality

Polyclonal

Myeloma

unknown

Isotype

IgG

Research areas

Neuroscience >> Neurotransmission >> Calcium Signaling >> Calcium Binding Proteins

  • Immunohistochemistry (Frozen sections) - Calretinin antibody (ab702)Immunohistochemistry (Frozen sections) - Calretinin antibody (ab702) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    IHC-P

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    IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.(
    For an ABC system, dilute 1/50-1/100 and incubate for 30-60 minutes at RT.)

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    IHC-Fr

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    IHC-Fr: 1/100(PubMed: 20504962

    Immunohistochemical studies have recently shown calretinin to be useful in distinguishing mesotheliomas from lung adenocarcinomas. However, it is recommended that a panel of antibodies be used in tandem with calretinin. Other antibodies recommended are CK 5/6, E-cadherin, ESA or BerEP4, CEA, EMA, B72.3, Vimentin and CD15. Calretinin does not mark all mesotheliomas (50-70%).)

Target

Function

Calretinin is a calcium-binding protein which is abundant in auditory neurons.

Tissue specificity

Brain.

Sequence similarities

Belongs to the calbindin family.
Contains 6 EF-hand domains.

Target information above from: UniProt accessionP22676 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 29 kDa calbindin antibody
  • CAB 29 antibody
  • CAB29 antibody
  • CAB29 antibody
  • CAL 2 antibody
  • CAL2 antibody
  • CAL2 antibody
  • CALB 2 antibody
  • CALB2 antibody
  • CALB2 antibody
  • CALB2_HUMAN antibody
  • Calbindin 2 29kDa antibody
  • Calbindin 2 29kDa antibody
  • Calbindin 2 antibody
  • Calbindin D29K antibody
  • Calbindin D29K antibody
  • Calbindin2 antibody
  • Calretinin antibody
  • CR antibody
  • CR antibody
see all

Database links

see all

Anti-Calretinin antibody images:

  Immunohistochemistry (Frozen sections) - Calretinin antibody (ab702)

Immunohistochemistry (Frozen sections) - Calretinin antibody (ab702)

ab702 staining Calretinin (green) in zebrafish retinal cells by Immunohistochemistry (frozen sections).

Embryos were first protected by sequential incubation in 15% then 30% sucrose in phosphate-buffered saline supplemented with 0.5% Triton X-100 (PBST) for 12-16 hours at 4°C, then embedded in OCT, stored at –80°C, and sectioned at 16-20 µm using a cryostat.

Embryos subjected to whole-mount in situ hybridisation were cleared in serial incubations of glycerol (25, 50, 75 and 95%), the eyes dissected and placed in a drop of glycerol then cover-slipped.

Original article: http://dev.biologists.org/content/137/13/2107.long

Adapted with permission from Cerveny KL et al, Development. 2010 Jul;137(13):2107-15. Epub 2010 May 26, Fig S1.

References for Anti-Calretinin antibody (ab702)

This product has been referenced in:

  • Jacquet BVet al. Specification of a Foxj1-Dependent Lineage in the Forebrain Is Required for Embryonic-to-Postnatal Transition of Neurogenesis in the Olfactory Bulb. J Neurosci 31:9368-82 (2011).Read more (PubMed: 21697387) »
  • Guic MMet al. Characterization of spinal afferent neurons projecting to different chambers of the rat heart. Neurosci Lett 469:314-8 (2010).Read more (PubMed: 20018227) »

See all 8 publications for this product

Publishing research using ab702? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"