Anti-Calretinin 抗体 [SP13] (ab16694)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP13] to Calretinin
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Calretinin antibody [SP13]
Calretinin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [SP13] to Calretinin -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, IHC-Frmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant full length protein within Mouse Calretinin. The exact sequence is proprietary.
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ポジティブ・コントロール
- IHC-P: Human mesothelioma. IHC-Fr: Mouse and rat cerebrum tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, 98.9% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
SP13 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16694の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P | (1) |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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IHC-Fr |
1/30.
|
特記事項 |
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IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
IHC-Fr
1/30. |
ターゲット情報
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機能
Calretinin is a calcium-binding protein which is abundant in auditory neurons. -
組織特異性
Brain. -
配列類似性
Belongs to the calbindin family.
Contains 6 EF-hand domains. - Information by UniProt
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参照データベース
- Entrez Gene: 794 Human
- Entrez Gene: 12308 Mouse
- Entrez Gene: 117059 Rat
- Omim: 114051 Human
- SwissProt: P22676 Human
- SwissProt: Q08331 Mouse
- SwissProt: P47728 Rat
- Unigene: 106857 Human
see all -
別名
- 29 kDa calbindin antibody
- CAB 29 antibody
- CAB29 antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded Human mesothelioma labeling Calretinin with ab16694 at 1/100 (27 µg/ml). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16694 for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. DAB was used as the chromogen. Counterstained with Hematoxylin and mounted with DPX.
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This image was generated using a previous batch manufactured using hybridoma production method.
Immunohistochemistry (Frozen) analysis of mouse cerebrum tissue section labeling Calretinin with purified ab16694 at 1/30 (9.6 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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This image was generated using a previous batch manufactured using hybridoma production method.
Immunohistochemistry (Frozen) analysis of rat cerebrum tissue section labeling Calretinin with purified ab16694 at 1/30 (9.6 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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This image was generated using a previous batch manufactured using hybridoma production method.
Immunohistochemistry (Frozen) analysis of rat cerebrum tissue section labeling Calretinin with purified ab16694 at 1/30 (9.6 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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This image was generated using a previous batch manufactured using hybridoma production method.
Immunohistochemistry (Frozen) analysis of mouse cerebrum tissue section labeling Calretinin with purified ab16694 at 1/30 (9.6 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab16694 は 10 報の論文で使用されています。
- Shi CJ et al. TGFβR-1/ALK5 inhibitor RepSox induces enteric glia-to-neuron transition and influences gastrointestinal mobility in adult mice. Acta Pharmacol Sin 44:92-104 (2023). PubMed: 35794374
- Pruett N et al. Normal mesothelial cell lines newly derived from human pleural biopsy explants. Am J Physiol Lung Cell Mol Physiol 319:L652-L660 (2020). PubMed: 32726133
- Murayama AY et al. The polymicrogyria-associated GPR56 promoter preferentially drives gene expression in developing GABAergic neurons in common marmosets. Sci Rep 10:21516 (2020). PubMed: 33299078
- Sakaguchi H et al. Self-Organized Synchronous Calcium Transients in a Cultured Human Neural Network Derived from Cerebral Organoids. Stem Cell Reports 13:458-473 (2019). PubMed: 31257131
- Ohara Y et al. Connective tissue growth factor-specific monoclonal antibody inhibits growth of malignant mesothelioma in an orthotopic mouse model. Oncotarget 9:18494-18509 (2018). PubMed: 29719620
- Yin M et al. Gremlin-1 is a key regulator of the invasive cell phenotype in mesothelioma. Oncotarget 8:98280-98297 (2017). PubMed: 29228689
- Roulois D et al. Characterization of preneoplastic and neoplastic rat mesothelial cell lines: the involvement of TETs, DNMTs, and 5-hydroxymethylcytosine. Oncotarget 7:34664-87 (2016). PubMed: 27129173
- Aierken D et al. Rat model demonstrates a high risk of tremolite but a low risk of anthophyllite for mesothelial carcinogenesis. Nagoya J Med Sci 76:149-60 (2014). PubMed: 25130001
- Lin B et al. Forebrain ischemia triggers GABAergic system degeneration in substantia nigra at chronic stages in rats. Cardiovasc Psychiatry Neurol 2010:506952 (2010). IHC ; Rat . PubMed: 20981346
- Kuwabara T et al. Wnt-mediated activation of NeuroD1 and retro-elements during adult neurogenesis. Nat Neurosci 12:1097-105 (2009). ICC/IF ; Rat . PubMed: 19701198