Application
Immunocytochemistry
Sample
Mouse Cultured Cells (thoracic aortic smooth muscle cells)
Permeabilization
Yes - 0.25% TritonX-100
Specification
thoracic aortic smooth muscle cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Additional data
Additional Notes
VSMCs were seeded to 35-mm plates in a low density avoiding overlapping of cells.
After fixation, VSMCs were treated with 0.25% TritonX-100 for 20 mins.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Mengcheng Shen
Verified customer
投稿 Dec 15 2014