Anti-Calpain 1 抗体 (ab39170)
Key features and details
- Rabbit polyclonal to Calpain 1
- Suitable for: IHC (PFA fixed), ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Calpain 1 antibody
Calpain 1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Calpain 1 -
由来種
Rabbit -
特異性
This antibody binds to reduced and non reduced Calpain 1. It reacts with Domain IV of Calpain 1, and detects both latent and aminoprocessed (activated) Calpain 1. It but does not cross react with the other calpain family members (Calpain 2, calpain 94, ncl 2, ncl 3, etc.). -
アプリケーション
適用あり: IHC (PFA fixed), ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide based on Domain IV of the large subunit of Human Calpain 1. (Peptide available as ab41300.)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab39170の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC (PFA fixed) |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 82 kDa.
1/1000 - 1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 82 kDa). Dilution optimised using Chromogenic detection. |
特記事項 |
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IHC (PFA fixed)
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 82 kDa. 1/1000 - 1/5000. Detects a band of approximately 75 kDa (predicted molecular weight: 82 kDa). Dilution optimised using Chromogenic detection. |
ターゲット情報
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機能
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 4 EF-hand domains. -
細胞内局在
Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding. - Information by UniProt
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参照データベース
- Entrez Gene: 823 Human
- Entrez Gene: 12333 Mouse
- Entrez Gene: 29153 Rat
- Omim: 114220 Human
- SwissProt: P07384 Human
- SwissProt: O35350 Mouse
- SwissProt: P97571 Rat
- Unigene: 502842 Human
see all -
別名
- Ca2 activated neutral protease antibody
- Calcium activated neutral proteinase antibody
- Calcium activated neutral proteinase small subunit antibody
see all
画像
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ICC/IF image of ab39170 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39170, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (32)
ab39170 は 32 報の論文で使用されています。
- Souza-Neto FV et al. Mitochondrial Oxidative Stress Promotes Cardiac Remodeling in Myocardial Infarction through the Activation of Endoplasmic Reticulum Stress. Antioxidants (Basel) 11:N/A (2022). PubMed: 35883722
- Muniappan L et al. Inducible Depletion of Calpain-2 Mitigates Abdominal Aortic Aneurysm in Mice. Arterioscler Thromb Vasc Biol 41:1694-1709 (2021). PubMed: 33761765
- Bensaada I et al. Calpastatin prevents Angiotensin II-mediated podocyte injury through maintenance of autophagy. Kidney Int 100:90-106 (2021). PubMed: 33675847
- Singh A et al. Enhanced tumor cell killing by ultrasound after microtubule depolymerization. Bioeng Transl Med 6:e10233 (2021). PubMed: 34589605
- Weber JJ et al. Calpain-1 ablation partially rescues disease-associated hallmarks in models of Machado-Joseph disease. Hum Mol Genet 29:892-906 (2020). PubMed: 31960910