Application
Western blot
Sample
Sheep Cell lysate - other (total protein, mitochondria and ER isolates)
Gel Running Conditions
Reduced Denaturing (10% PAG)
Loading amount
10 µg
Specification
total protein, mitochondria and ER isolates
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% milk
Dilution
1/800
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: goat anti-rabbit igg HRP conjugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
mitochondria isolated from preadipocytes
Exposure
30 second(s)
Bands
Specific: 90 kDa
Positive control
endoplasmic reticulum isolated from preadipocytes
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MISS. Barbara Makela
Verified customer
投稿 Mar 08 2017