Application
Western blot
Sample
Human Cell lysate - nuclear (HeLa)
Loading amount
20 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-0.1% Tween
Secondary antibody
Name
Non-Abcam antibody was used: goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/30000
Detection
Detection method
ECL Advance
Exposure
20 second(s)
Bands
Specific: 17 kDa Non-specific: 38, 41, 56, 76 kDa
Positive control
whole cell lysate and nuclear extract
Additional data
Additional Notes
Lane 1 = whole cell lysate
Lane 2 = nuclear extract
Lane 2 = nuclear extract
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 18 2013