Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (ES Cells and Neural cells)
Permeabilization
Yes - 0.4%-1% Triton
Specification
ES Cells and Neural cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking buffer
Dilution
1/100
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Alexa Flour Goat anti-Rabbit 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Abcam response
We would like to thank the reviewer for submitting an Abreview for this product.
As the product had not yet been tested in ICC/IF applications, we were unsure what the result might be; we appreciate the time and effort that the reviewer has put in to testing this product in this application.
Further optimization may be necessary for use of this product in ICC/IF applications, and we are happy to help troubleshoot any products or applications which may not be performing.
We do encourage all of our customers to contact us should they wish to test any of our products in untested species or applications, as those products may qualify for our AbTrial Testing Program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Maneeshi Prasad
Verified customer
投稿 Jul 21 2016