Application
Western blot
Sample
Rat Tissue lysate - whole (Rat Artery (Mesenteric/Cerebral/Renal))
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
30 µg
Specification
Rat Artery (Mesenteric/Cerebral/Renal)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% NFSM
Dilution
1/2000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Anti-Rabbiit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 50-55 kDa
Additional data
Additional Notes
This antibody performs well producing two strong bands (same as competition) The two fainter bands above are all also seen. One issue is that this primary antibody binds to the NEB P7712S marker horrendously badly. This is not seen with other primaries using the same secondary.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Edward Humphries
Verified customer
投稿 May 24 2017