Anti-CYP3A43 抗体 (ab155029)
Key features and details
- Rabbit polyclonal to CYP3A43
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CYP3A43 antibody
CYP3A43 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CYP3A43 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Monkey -
免疫原
Recombinant fragment corresponding to a region within amino acids 33-248 of Human CYP3A43 (UniProt ID: Q9HB55).
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ポジティブ・コントロール
- HCT116, HepG2, HeLa and A549 whole cell lysate. HeLa cells. Human breast carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab155029の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 58 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 58 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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関連性
CYP3A43 is a member of the cytochrome P450 superfamily of enzymes which are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. CYP3A43 exhibits low testosterone 6 beta hydroxylase activity. -
細胞内局在
Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. -
参照データベース
- Entrez Gene: 64816 Human
- Omim: 606534 Human
- SwissProt: Q9HB55 Human
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別名
- Cytochrome P450 family 3 subfamily A polypeptide 43 antibody
- Cytochrome P450 polypeptide 43 antibody
- Cytochrome P450 subfamily IIIA polypeptide 43 antibody
see all
画像
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Anti-CYP3A43 antibody (ab155029) at 1/2000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 58 kDa
10% SDS PAGE -
Immunofluorescence analysis of methanol fixed HeLa cells, labeling CYP3A43 using ab155029 at 1/200 dilution. Lower image costained with Hoechst 33342.
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Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue, labeling CYP3A43 using ab155029 at 1/250 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab155029 は 5 報の論文で使用されています。
- Wang L et al. MicroRNA-519c-3p promotes tumor growth and metastasis of hepatocellular carcinoma by targeting BTG3. Biomed Pharmacother 118:109267 (2019). PubMed: 31387005
- Reebye V et al. Gene activation of CEBPA using saRNA: preclinical studies of the first in human saRNA drug candidate for liver cancer. Oncogene 37:3216-3228 (2018). PubMed: 29511346
- Meng D et al. MicroRNA-645 targets urokinase plasminogen activator and decreases the invasive growth of MDA-MB-231 triple-negative breast cancer cells. Onco Targets Ther 11:7733-7743 (2018). PubMed: 30464522
- Li J et al. MicroRNA-140-3p enhances the sensitivity of hepatocellular carcinoma cells to sorafenib by targeting pregnenolone X receptor. Onco Targets Ther 11:5885-5894 (2018). PubMed: 30271172
- Richter M et al. Pancreatic progenitor-derived hepatocytes are viable and functional in a 3D high density bioreactor culture system. Toxicol Res (Camb) 5:278-290 (2016). PubMed: 30090344