Anti-CYP2R1 抗体 (ab137634)
Key features and details
- Rabbit polyclonal to CYP2R1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CYP2R1 antibody
CYP2R1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CYP2R1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IF, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Monkey -
免疫原
Recombinant fragment corresponding to a region within amino acids 83 and 360 of Human CYP2R1.
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ポジティブ・コントロール
- HeLa whole cell lysate; methanol-fixed HeLa cells; FaDu xenograft.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab137634の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 57 kDa.
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特記事項 |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 57 kDa. |
ターゲット情報
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機能
Has a D-25-hydroxylase activity on both forms of vitamin D, vitamin D(2) and D(3). -
関連疾患
Defects in CYP2R1 are the cause of rickets vitamin D-dependent type 1B (VDDR1B) [MIM:600081]; also known as pseudovitamin D(3) deficiency rickets due to 25-hydroxylase deficiency. A disorder caused by a selective deficiency of the active form of vitamin D (1,25-dihydroxyvitamin D3) and resulting in defective bone mineralization and clinical features of rickets. The patients sera have low calcium concentrations, low phosphate concentrations, elevated alkaline phosphatase activityand low levels of 25-hydroxyvitamin D. -
配列類似性
Belongs to the cytochrome P450 family. -
細胞内局在
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 120227 Human
- Entrez Gene: 244209 Mouse
- Omim: 608713 Human
- SwissProt: Q6VVX0 Human
- SwissProt: Q6VVW9 Mouse
- Unigene: 371427 Human
- Unigene: 108037 Mouse
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別名
- CP2R1_HUMAN antibody
- CYP2R1 antibody
- Cytochrome P450 2R1 antibody
see all
画像
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Anti-CYP2R1 antibody (ab137634) at 1/3000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 57 kDa
7.5% SDS Page -
Immunohistochemical analysis of paraffin-embedded FaDu xenograft, labelling CYP2R1 using ab137634 at 1/500 dilution.
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Immunofluorescence analysis of methanol-fixed HeLa cells, labelling CYP2R1 using ab137634 at 1/200 dilution. lower panel represents cells costained with Hoechst 33342.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab137634 は 3 報の論文で使用されています。
- Anisiewicz A et al. Vitamin D Metabolite Profile in Cholecalciferol- or Calcitriol-Supplemented Healthy and Mammary Gland Tumor-Bearing Mice. Nutrients 12:N/A (2020). PubMed: 33172201
- Bukuroshi P et al. Strategies and limitations associated with in vitro characterization of vitamin D receptor activators. Biochem Pharmacol 155:547-561 (2018). PubMed: 30028992
- Bergadà L et al. Role of local bioactivation of vitamin D by CYP27A1 and CYP2R1 in the control of cell growth in normal endometrium and endometrial carcinoma. Lab Invest 94:608-22 (2014). PubMed: 24732451