Anti-CYP27A1 抗体 [EPR7529] - BSA and Azide free (ab214800)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7529] to CYP27A1 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CYP27A1 antibody [EPR7529] - BSA and Azide free
CYP27A1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7529] to CYP27A1 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- HepG2, Caco 2, Human fetal liver and Mouse liver lysates; Human kidney and liver tissues; HepG2 cells.
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特記事項
ab214800 is the carrier-free version of ab126785.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
解離定数(KD 値)
KD = 6.00 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7529 -
アイソタイプ
IgG -
研究分野
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
関連製品
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Alternative Versions
- Anti-CYP27A1 antibody [EPR7529] (ab126785)
- PE Anti-CYP27A1 antibody [EPR7529] (ab305862)
- APC Anti-CYP27A1 antibody [EPR7529] (ab305863)
- HRP Anti-CYP27A1 antibody [EPR7529] (ab305864)
- Alexa Fluor® 488 Anti-CYP27A1 antibody [EPR7529] (ab309770)
- Alexa Fluor® 647 Anti-CYP27A1 antibody [EPR7529] (ab310141)
- Alexa Fluor® 594 Anti-CYP27A1 antibody [EPR7529] (ab310565)
- Alexa Fluor® 555 Anti-CYP27A1 antibody [EPR7529] (ab312094)
- Alexa Fluor® 568 Anti-CYP27A1 antibody [EPR7529] (ab312574)
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab214800の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 60 kDa.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use at an assay dependent concentration.
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特記事項 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 60 kDa. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity. -
パスウェイ
Hormone biosynthesis; cholecalciferol biosynthesis. -
関連疾患
Cerebrotendinous xanthomatosis -
配列類似性
Belongs to the cytochrome P450 family. -
細胞内局在
Mitochondrion membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1593 Human
- Entrez Gene: 104086 Mouse
- Entrez Gene: 301517 Rat
- Omim: 606530 Human
- SwissProt: Q02318 Human
- SwissProt: Q9DBG1 Mouse
- SwissProt: P17178 Rat
- Unigene: 516700 Human
see all -
別名
- 12-alpha-triol 27-hydroxylase antibody
- 5-beta-cholestane-3-alpha antibody
- 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase antibody
see all
画像
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ab126785, at a 1/100 dilution, staining CYP27A1 in paraffin embedded Human kidney tissue by Immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126785).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Intracellular Flow Cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling CYP27A1 (red) with ab126785 at a 1/120 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126785).
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ab126785, at a 1/100 dilution, staining CYP27A1 in paraffin embedded Human liver tissue by Immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126785).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KDThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126785).
プロトコール
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (2)
ab214800 は 2 報の論文で使用されています。
- Soffientini U et al. Intracellular cholesterol transporters and modulation of hepatic lipid metabolism: Implications for diabetic dyslipidaemia and steatosis. Biochim Biophys Acta 1842:1372-82 (2014). PubMed: 25014273
- Aleksunes LM et al. Repression of hepatobiliary transporters and differential regulation of classic and alternative bile acid pathways in mice during pregnancy. Toxicol Sci 130:257-68 (2012). WB ; Mouse . PubMed: 22903823