製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CYB5R4
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 357-508 of Human CYB5R4 (AAH25380).

  • ポジティブ・コントロール
    • NT2D1, IMR32 and U87-MG cell lines.

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab101342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 59 kDa.

ターゲット情報

  • 機能
    NADH-cytochrome b5 reductase involved in endolasmic reticulum stress response pathway. Plays a critical role in protecting pancreatic beta-cells against oxidant stress, possibly by protecting the cell from excess buildup of reactive oxygen species (ROS). Reduces a variety of substrates in vitro, such as cytochrome c, feericyanide and methemoglobin.
  • 組織特異性
    Widely expressed.
  • 配列類似性
    Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
    Contains 1 CS domain.
    Contains 1 cytochrome b5 heme-binding domain.
    Contains 1 FAD-binding FR-type domain.
  • 細胞内局在
    Endoplasmic reticulum. Soluble protein.
  • Information by UniProt
  • 参照データベース
  • 別名
    • b5+b5R antibody
    • b5b5r antibody
    • C79736 antibody
    • cb5/cb5R antibody
    • Cb5cb5r antibody
    • Cyb5r4 antibody
    • Cytochrome b type NAD(P)H oxidoreductase antibody
    • Cytochrome b5 reductase 4 antibody
    • dJ676J13.1 antibody
    • Flavohemoprotein b5+b5R antibody
    • Flavohemoprotein b5/b5R antibody
    • N terminal cytochrome b5 and cytochrome b5 oxidoreductase domain containing protein antibody
    • N-terminal cytochrome b5 and cytochrome b5 oxidoreductase domain-containing protein antibody
    • NADPH cytochrome B5 oxidoreductase antibody
    • NB5R4_HUMAN antibody
    • NCB5OR antibody
    • RP4 676J13.1 antibody
    see all

画像

  • Anti-CYB5R4 antibody (ab101342) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg

    Predicted band size : 59 kDa

プロトコール

参考文献

ab101342 has not yet been referenced specifically in any publications.

レビューと Q&A

Abreviews
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-15%)
Sample
Mouse Tissue lysate - whole (Liver)
Specification
Liver
Treatment
1 X SDS Loading Buffer
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jan 17 2014

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
2% BSA/2% Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Whole Brain Sagittal)
Specification
Whole Brain Sagittal
Permeabilization
Yes - 0.1% Triton
Fixative
Paraformaldehyde
Username

Mr. Matt Stroh

Verified customer

投稿 Jan 15 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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