Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (spleen, lymphe nodes)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citratbuffer pH6.0 / Tris-EDTA pH 9.0
Permeabilization
No
Specification
spleen, lymphe nodes
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-Tween 0.05%
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: LSAB-Kit DAKO K5003
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Abcam response
Thank you for your review in this untested species and application. To improve your results you may try increasing the amount of antibody used for staining and switching to a high pH antigen retrieval buffer. These steps often result in stronger signal.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Aug 14 2015