Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (gel 10%)
Sample
Mouse Cell lysate - whole cell (Epithelial ovarian cancer cell line)
Specification
Epithelial ovarian cancer cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 4°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: overnight
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
Chemiluminescence
Bands
Specific: 50 kDa Non-specific: 35-15 kDa
Exposure
30 second(s)
Positive control
ID8 mouse epithelial ovarian cancer cell line and SKOV-3 Human epithelial ovarian cancer cell line
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Hilal Gurler
Verified customer
投稿 Jun 18 2013