Application
ELISA
Sample
Human Recombinant protein (Recombinant Human CX3CL1 protein, ab87480)
Specification
Recombinant Human CX3CL1 protein, ab87480
Type
Sandwich (Capture)
Other product details
Incubation time
15 minute(s) · Temperature: 25°C · Diluent: PBST
Concentration
0.75 µg/ml
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
It's not a conventional ELISA, the antibodies were used for developing an immunoassay using magnetic particles with streptavidin (Dynabeads™ M-280 Streptavidin (Thermo-Fisher))
https://www.thermofisher.com/order/catalog/product/11205D
The antibody Ab9819 was used as secondary antibody. This secondary antibody (Ab9819) was conjugated with HRP using HRP conjugation kit (ab102890) by the researchers. The Ab9819 concentration used was 0.83 ´g/ml.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 15 2017