Application
Immunohistochemistry (Frozen sections)
Sample
Zebra finch Tissue sections (embryonal forebrain)
Specification
embryonal forebrain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% TX100 in PBS
Blocking step
Roti-Immunoblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Other product details
Dilution
1/100
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1:100 Roti-Immunoblock
Secondary antibody
Name
Non-Abcam antibody was used: Vector labs BA1000, affinity purified
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
specimen: Zebra finch embryonal forebrain, 16µm kryosections
fixation: postfixed in 4% fresh PFA for 15 min at RT
also tested with different blocking reagents:similarly inconclusive
-5% HSerum for blocking, 1%Hserum for antibody incubation
fixation: postfixed in 4% fresh PFA for 15 min at RT
also tested with different blocking reagents:similarly inconclusive
-5% HSerum for blocking, 1%Hserum for antibody incubation
Abcam response
Thank you for your valuable feedback. We are sorry to hear that this antibody has not given you good results in zebra fish and immunohistochemistry with frozen tissue section (IHC-Fr). This antibody has not been tested in this species and application before, and it is possible that it is not suitable for use, or the vial you received may have been damaged in some way. However, we would be unable to confirm this without further testing of the antibody in zebra fish and IHC-Fr application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Robert Storm
Verified customer
投稿 Feb 06 2012