Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse ES cells)
Loading amount
100000 cells
Specification
mouse ES cells
Treatment
72 hours after transfection with Silencer® Select Pre-designed siRNA against Ctr9 (s75508)
Gel Running Conditions
Non-reduced Denaturing (Invitrogen NuPage 4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/3000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% milk in PBS_0.1% Tween 20
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG (H+L) HRP conjugated Conjugat
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000
Detection
Detection method
SuperSignal West Femto Maximum Sensitivi
Exposure
10 second(s)
Bands
Specific: 135 kDa
Positive control
RNAi knockdown using Silencer® Select Pre-designed siRNA against Ctr9 (s75508)
Negative control
Mock transfection
Additional data
Additional Notes
Correct size (around 135 kDa), Very specific (only one band) and strong signal.
Clear decrease of band signal after transfection using Silencer® Select Pre-designed siRNA against Ctr9 (s75508), compared to control samples
Clear decrease of band signal after transfection using Silencer® Select Pre-designed siRNA against Ctr9 (s75508), compared to control samples
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 22 2010