Anti-CTCF 抗体 (ab70303)
Key features and details
- Rabbit polyclonal to CTCF
- Suitable for: IHC-Fr, WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-CTCF antibody
CTCF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CTCF -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Fr, WB, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
免疫原
Synthetic peptide corresponding to Human CTCF aa 650-750.
Database link: P49711 -
ポジティブ・コントロール
- IP: Jurkat whole cell lysate. IHC-P: Human lung cancer tissue, mouse renal cancer tissue. WB: Jurkat, HEK293T and HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab70303 was affinity purified using an epitope specific to CTCF immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70303の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-Fr | (1) |
Use at an assay dependent concentration.
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WB | (5) |
1/2500 - 1/10000.
Detects a band of approximately 100 kDa (predicted molecular weight: 83 kDa). |
IP |
Use at 2-5 µg/mg of lysate.
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IHC-P | (1) |
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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IHC-Fr
Use at an assay dependent concentration. |
WB
1/2500 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 83 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates to the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays a important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping. Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X- inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19. -
組織特異性
Ubiquitous. Absent in primary spermatocytes. -
配列類似性
Belongs to the CTCF zinc-finger protein family.
Contains 11 C2H2-type zinc fingers. -
ドメイン
The 11 zinc fingers are highly conserved among vertebrates, exhibiting almost identical amino acid sequences. Different subsets or combination of individual zinc fingers gives the ability to CTCF to recognize multiple DNA target sites. -
翻訳後修飾
Sumoylated on Lys-74 and Lys-689; sumoylation of CTCF contributes to the repressive function of CTCF on the MYC P2 promoter. -
細胞内局在
Nucleus > nucleoplasm. Chromosome. Chromosome > centromere. May translocate to the nucleolus upon cell differentiation. Associates with both centromeres and chromosomal arms during metaphase. Associates with the H19 ICR in mitotic chromosomes. May be preferentially excluded from heterochromatin during interphase. - Information by UniProt
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参照データベース
- Entrez Gene: 517097 Cow
- Entrez Gene: 10664 Human
- Entrez Gene: 13018 Mouse
- Entrez Gene: 100623274 Pig
- Entrez Gene: 83726 Rat
- Omim: 604167 Human
- SwissProt: P49711 Human
- SwissProt: Q61164 Mouse
see all -
別名
- 11 zinc finger protein antibody
- 11 zinc finger transcriptional repressor antibody
- 11-zinc finger protein antibody
see all
画像
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CTCF was immunoprecipitated from 1 mg Jurkat whole cell lysate with ab70303 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab70303 at 0.02 µg/mL.
Lane 1: ab70303 IP in Jurkat whole cell lysate.
Lane 2: Contol IgG in Jurkat whole cell lysate.
Detection: Chemiluminescence.
Exposure time: 10 seconds.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human small cell lung cancer tissue, labeling CTCF with ab70303 at a 1/1000 dilution. HRP-conjugated goat anti-rabbit IgG was used as a secondary antibody.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse renal cancer tissue, labeling CTCF with ab70303 at a 1/1000 dilution. HRP-conjugated goat anti-rabbit IgG was used as a secondary antibody.
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All lanes : Anti-CTCF antibody (ab70303) at 0.02 µg/ml
Lane 1 : Jurkat whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 50 µg per lane.
Exposure time: 10 secondsDetection: Chemiluminescence.Lysates prepared using NETN lysis buffer.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (75)
ab70303 は 75 報の論文で使用されています。
- Tian GG et al. Integrative analysis of the 3D genome structure reveals that CTCF maintains the properties of mouse female germline stem cells. Cell Mol Life Sci 79:22 (2022). PubMed: 34981210
- Haarhuis JHI et al. A Mediator-cohesin axis controls heterochromatin domain formation. Nat Commun 13:754 (2022). PubMed: 35136067
- Aljahani A et al. Analysis of sub-kilobase chromatin topology reveals nano-scale regulatory interactions with variable dependence on cohesin and CTCF. Nat Commun 13:2139 (2022). PubMed: 35440598
- Di Giammartino DC et al. Assessing Specific Networks of Chromatin Interactions with HiChIP. Methods Mol Biol 2532:113-141 (2022). PubMed: 35867248
- Zelenka T et al. The 3D enhancer network of the developing T cell genome is shaped by SATB1. Nat Commun 13:6954 (2022). PubMed: 36376298