CRMP4 antibody (ab23951)

Product Name 

CRMP4 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to CRMP4

Immunogen 

Synthetic peptide: YDGPVFDLTT TPK, corresponding to C terminal amino acids 499-511 of Human CRMP4
BLAST 'YDGPVFDLTT TPK' with or

Reacts with 
(species key)

Hu, Ms, Rat, Cat, Mk

Specificity 

CRMP4 protein (Ulip1/TUC4). This antibody has been reported to label early postmitotic neurons in prenatal rat brain.   The antibody is not reactive with the proteins TUC-1 (Ulip3/CRMP1), TUC2 (Ulip2/CRMP2) or TUC3 (Ulip4/CRMP3). It does not label neural progenitor or radial glia.

Tested applications 
(see key)

ICC, IHC-FoFr, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC: 1/5000 - 1/10000 (DAB detection).
IHC-Fo: 1/5000 - 1/10000 using PFA (DAB detection).
IP: Use at an assay dependent dilution.
WB: 1/10000 - 1/100000 using alkaline phosphatase. Predicted molecular weight: 62 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Mainly in heart and skeletal muscle. Also expressed strongly in fetal brain and spinal cord.

Cellular localization 

Cytoplasmic. Cell projection, growth cone (By similarity). Note: Colocalizes with synaptic vesicle protein 2 in the central region of the growth cone.

Research areas 

Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other

Relevance 

The collapsin response mediator protein (CRMP) family of five cytosolic phosphoproteins are highly expressed throughout brain development. The functions of CRMPs encompass signal transduction in developmental guidance cues as well as multiple cellular and molecular events involved in apoptosis/proliferation, cell migration, and differentiation. In the adult brain, the expression of CRMPs is dramatically downregulated. However, CRMPs remain expressed in structures that retain their capacity for differentiation and plasticity. The expression of CRMPs is altered in neurodegenerative diseases, and these proteins may have a role in the physiopathology of the adult nervous system.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: 0.2% Sodium Azide
Constituents: Whole serum

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

この製品は、毒物及び劇物取締法に基づく「毒物」に該当します。

Form 

Liquid

Concentration 

Concentration not determined - その理由

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

References for CRMP4 antibody (ab23951)

  This product has been used in: (two most recent references)

Yocum AK et al. Coupled global and targeted proteomics of human embryonic stem cells during induced differentiation. Mol Cell Proteomics 7:750-67 (2008). PubMed: 18304949

Gramolini AO et al. Comparative proteomic profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses. Mol Cell Proteomics : (2007). WB; Mouse. PubMed: 18056057
If you publish research using ab23951 please let us know so that we can cite the reference on this datasheet.

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