Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing
Sample
Cow Tissue lysate - whole (Subcutaneous Adipose)
Specification
Subcutaneous Adipose
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Li-Cor IRDye 800
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Detection
Detection method
Li-Cor imager
Bands
Specific: 43 kDa Non-specific: 64 kDa
Exposure
5 minute(s) and 0 second(s)
Positive control
3rd lane: mouse liver protein, 4th lane: mouse muscle protein
Additional data
Abcam response
Thank you so much for testing this antibody in Bovine.
We appreciate very much your efforts, but are sorry to hear that the antibody did not work optimally in this species). The customer received a discount on a future antibody purchase for testing this product in Bovine. We would welcome additional feedback from other customers as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 08 2013