Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step
None
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS-Triton (0.3%) + azide (0.02%)
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 568 donkey anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 568
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 568
Dilution
1/1000
Additional data
Additional Notes
The tissues were fixed (animals perfused fixed) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.
Immunostaining obtained with this antibody in the mouse cortex using the antibody 1:1000 diluted. The staining is beautiful, located in the nuclei of cells as expected.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Karine Thibault
Verified customer
投稿 Aug 19 2014